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Reconstitution & Dosing Guide

Step-by-step mixing instructions and research dosing protocols for every peptide we carry.

⚠️ Research Use Only. All compounds sold by Biohax Champions are for laboratory research purposes only. These protocols are provided as educational reference material. Not intended for human use, consumption, or medical treatment. Always follow applicable regulations in your jurisdiction.
Metabolic Peptides
🔥

RETA — Retatrutide

Triple GIP/GLP-1/Glucagon receptor agonist · 30mg Vial
Vial Size
30 mg
BAC Water to Add
3.0 mL
Resulting Concentration
10 mg/mL
Storage After Mixing
2–4 weeks at 2–8°C
Reconstitution Steps
  1. Wipe the vial stopper with an alcohol swab and let it air dry (~10 seconds).
  2. Draw 3.0 mL of bacteriostatic water into a sterile syringe.
  3. Insert needle and inject water slowly down the side wall of the vial — not directly onto the powder.
  4. Gently roll the vial between your palms for 30–60 seconds until fully dissolved.
  5. Inspect: solution should be clear and colorless. Discard if cloudy or particulate.
  6. Label the vial with the date and concentration (10 mg/mL). Refrigerate immediately.
Research Dosing Protocol
WeekDoseVolume (10 mg/mL)Frequency
1–41 mg0.10 mL (10 units)Once weekly
5–82 mg0.20 mL (20 units)Once weekly
9–124 mg0.40 mL (40 units)Once weekly
13–168 mg0.80 mL (80 units)Once weekly
17–21+12 mg1.20 mL (120 units)Once weekly
Do not freeze reconstituted solution. Subcutaneous injection into abdomen or thigh — rotate sites.
💉

SEMA — Semaglutide

Long-acting GLP-1 receptor agonist · 30mg Vial
Vial Size
30 mg
BAC Water to Add
3 mL
Resulting Concentration
10 mg/mL
Storage After Mixing
≤28 days at 2–8°C
Reconstitution Steps
  1. Clean the vial stopper with an alcohol swab. Let dry.
  2. Draw 3 mL of bacteriostatic water into a sterile syringe.
  3. Inject water slowly along the side wall of the vial — never directly onto the powder.
  4. Roll gently between palms 30–60 seconds. Do not shake.
  5. Solution should be clear and colorless. Discard if cloudy.
  6. Label with date and concentration (10 mg/mL). Refrigerate at 2–8°C.
Research Dosing Protocol
WeekDoseVolume (10 mg/mL)Frequency
1–40.25 mg0.025 mL (2.5 units)Once weekly
5–80.50 mg0.05 mL (5 units)Once weekly
9–121.0 mg0.10 mL (10 units)Once weekly
13–161.7 mg0.17 mL (17 units)Once weekly
17+2.4 mg0.24 mL (24 units)Once weekly
Do not freeze reconstituted solution. Inject subcutaneously — abdomen, thigh, or upper arm. Rotate sites each week.
⚗️

TESA — Tesamorelin

Stabilized GHRH analogue · 10mg Vial
Vial Size
10 mg
BAC Water to Add
2.0–3.0 mL
Resulting Concentration
3.3–5 mg/mL
Storage After Mixing
Up to 28 days at 2–8°C
Reconstitution Steps
  1. Wipe stopper with alcohol swab. Allow to air dry.
  2. For a 3.3 mg/mL solution: draw 3.0 mL BAC water. For 5 mg/mL: draw 2.0 mL.
  3. Inject water slowly down the inside wall of the vial.
  4. Roll gently 30–60 seconds until fully dissolved. Do not shake.
  5. Inspect for clarity. Discard if cloudy or discolored.
  6. Label with date and concentration. Refrigerate immediately.
Research Dosing Protocol
ProtocolDoseVolume (3.3 mg/mL)Frequency
Standard2 mg0.60 mL (60 units)Once daily
Lower-dose start1 mg0.30 mL (30 units)Once daily
No titration required — full dose from day one per research protocols. Inject subcutaneously into abdomen. Rotate injection sites daily.
Do not freeze reconstituted solution. Discard any cloudy or discolored solutions.
Blend Products
🌿
✦ Quad Blend — 80mg Total

KLOW

GHK-Cu 50mg + KPV 10mg + TB-500 10mg + BPC-157 10mg
GHK-Cu 50mg KPV 10mg TB-500 10mg BPC-157 10mg
Vial Size
80 mg total
BAC Water to Add
2.0 mL
Resulting Concentration
40 mg/mL blend
Storage After Mixing
2–3 weeks at 2–8°C
Reconstitution Steps
  1. Wipe the vial stopper with an alcohol swab. Let dry completely.
  2. Draw 2.0 mL of bacteriostatic water into a sterile syringe.
  3. Insert needle and inject water slowly down the side wall — never directly onto the powder.
  4. Roll vial gently between palms for 30–60 seconds until fully dissolved. Do not shake.
  5. Inspect solution. A faint blue tint is normal (from GHK-Cu copper complex). Discard if cloudy or has unusual particles.
  6. Label with date, concentration, and contents. Refrigerate immediately. Protect from light.
Research Dosing Protocol
PhaseDoseVolume (2 mL reconstitution)Frequency
Initiation (Week 1–2)0.5–1.0 mg BPC-157 equivalent0.10–0.25 mLOnce daily
Maintenance1.0 mg BPC-157 equivalent0.25 mL (25 units)Once or twice daily
This is a multi-peptide blend. Each component contributes to tissue repair (BPC-157, TB-500), collagen stimulation (GHK-Cu), and anti-inflammatory support (KPV). Inject subcutaneously near the target area when possible.
Do not freeze reconstituted solution. Protect from light. A faint blue tint from GHK-Cu copper complex is normal and does not indicate degradation.
⚔️
✦ Dual Blend — 20mg Total

WOLVERINE

BPC-157 10mg + TB-500 10mg
BPC-157 10mg TB-500 10mg
Vial Size
20 mg total
BAC Water to Add
2.0 mL
Resulting Concentration
10 mg/mL blend
Storage After Mixing
2–3 weeks at 2–8°C
Reconstitution Steps
  1. Wipe vial stopper with alcohol swab. Let dry.
  2. Draw 2.0 mL of bacteriostatic water into a sterile syringe.
  3. Inject water slowly down the inside wall. Do not spray directly onto powder.
  4. Gently swirl between palms until clear. Do not shake.
  5. Verify solution is clear and colorless. Discard if cloudy.
  6. Label with date and concentration. Refrigerate immediately.
Research Dosing Protocol
PhaseDoseVolume (2 mL reconstitution)Frequency
Loading (Weeks 1–6)2–2.5 mg0.40–0.50 mL (40–50 units)Twice weekly
Maintenance (Week 7+)2–2.5 mg0.40–0.50 mL (40–50 units)Once weekly
Synergistic tissue repair blend. BPC-157 works locally to regenerate tissue, while TB-500 provides systemic healing support. Inject near the site of injury or subcutaneously in the abdomen.
Do not freeze reconstituted solution. Avoid light exposure.
Individual Peptides
🔵

GHK-Cu

Copper peptide — collagen synthesis, tissue repair · 50mg Vial (in KLOW blend)
Vial Size
50 mg
BAC Water to Add
2.0 mL
Concentration
25 mg/mL
Storage After Mixing
Refrigerate, protect from light
Reconstitution Steps
  1. Wipe stopper with alcohol swab. Let dry.
  2. Draw 2.0 mL of bacteriostatic water.
  3. Inject slowly down the side wall of the vial.
  4. Roll gently between palms until dissolved.
  5. A faint blue tint is normal from the copper complex. Discard only if cloudy with unusual particles.
  6. Refrigerate and protect from light.
Research Dosing
RangeDoseVolume (25 mg/mL)Frequency
Conservative1.0 mg0.04 mL (4 units)Daily (5 on/2 off)
Standard1.0–1.5 mg0.04–0.06 mLDaily
Higher-end1.5–2.0 mg0.06–0.08 mLDaily
🩹

BPC-157

Body protection compound — gut, tendon & tissue repair · 10mg Vial (in KLOW & WOLVERINE)
Vial Size
10 mg
BAC Water to Add
2.0 mL
Concentration
5,000 mcg/mL
Storage After Mixing
3–4 weeks at 2–8°C
Reconstitution Steps
  1. Inspect the vial for integrity.
  2. Wipe both stoppers with alcohol swabs.
  3. Draw 2 mL of bacteriostatic water.
  4. Inject slowly down the vial wall — avoid direct contact with powder.
  5. Swirl gently between palms until the solution clears.
  6. Verify clarity. Refrigerate immediately at 2–8°C.
Research Dosing
RangeDoseVolume (5,000 mcg/mL)Frequency
Low250 mcg0.05 mL (5 units)Once daily
Standard250 mcg0.05 mL (5 units)Twice daily
High500 mcg0.10 mL (10 units)Twice daily
Inject subcutaneously near the injury site when possible for localized effects, or in the abdomen for systemic gut support.
💪

TB-500 / Thymosin Beta-4

Systemic tissue healing, muscle & tendon repair · 10mg Vial (in KLOW & WOLVERINE)
Vial Size
10 mg
BAC Water to Add
2.0 mL
Concentration
5 mg/mL
Storage After Mixing
2–4 weeks at 2–8°C
Reconstitution Steps
  1. Inspect the vial. Wipe both stoppers with alcohol swabs and let dry.
  2. Draw 2.0 mL of bacteriostatic water.
  3. Inject BAC water slowly down the inside wall of the vial.
  4. Gently swirl (do not shake) until the solution clears.
  5. Label with reconstitution date. Refrigerate. Avoid light exposure.
Research Dosing
PhaseDoseVolume (5 mg/mL)Frequency
Loading (Weeks 1–6)2.0–2.5 mg0.40–0.50 mL (40–50 units)Twice weekly
Maintenance (Week 7+)2.0–2.5 mg0.40–0.50 mL (40–50 units)Once weekly
🛡️

KPV

Anti-inflammatory tripeptide — gut & mucosal support · 10mg Vial (in KLOW blend)
Vial Size
10 mg
BAC Water to Add
2.0 mL
Concentration
5,000 mcg/mL
Storage After Mixing
Up to 30 days at 2–8°C
Reconstitution Steps
  1. Wipe stopper with alcohol swab. Let dry.
  2. Draw 2.0 mL of bacteriostatic water.
  3. Add water slowly to the vial along the inside wall.
  4. Roll gently between palms for 30–60 seconds. Avoid shaking.
  5. Inspect for clarity. Discard if cloudy.
  6. Label with date and concentration. Refrigerate.
Research Dosing
PhaseDoseVolume (5,000 mcg/mL)Frequency
Initiation200 mcg0.04 mL (4 units)Once daily
Titration300–400 mcg0.06–0.08 mLOnce daily
Maintenance400–500 mcg0.08–0.10 mL (10 units)Once daily
GH Axis Peptides
📈
✦ Synergistic Blend

CJC / Ipamorelin

GHRH + ghrelin receptor agonist blend — pulsatile GH secretion · 10mg Vial
Vial Size
10 mg
BAC Water to Add
2.0 mL
Concentration
5 mg/mL
Storage After Mixing
2–4 weeks at 2–8°C
Reconstitution Steps
  1. Wipe the vial stopper with an alcohol swab. Allow to dry.
  2. Draw 2.0 mL of bacteriostatic water into a sterile syringe.
  3. Inject water slowly down the inside wall of the vial.
  4. Gently roll between palms until completely dissolved. Do not shake.
  5. Solution should be clear and colorless. Discard if cloudy.
  6. Label with date and concentration. Refrigerate at 2–8°C.
Research Dosing Protocol
DoseVolume (5 mg/mL)TimingFrequency
200–300 mcg0.04–0.06 mL (4–6 units)Fasted state, pre-bed or morningOnce to twice daily
Inject at least 2 hours after eating and before sleep for best results per research protocols. The two peptides work synergistically — CJC stimulates GH release while Ipamorelin amplifies the pulse with minimal cortisol impact.
Avoid injecting within 2 hours of a meal — food blunts GH release. Do not freeze reconstituted solution.
⚡

CJC No DAC / Modified GRF 1-29

Short-acting GHRH analogue — natural pulsatile GH release · 10mg Vial
Vial Size
10 mg
BAC Water to Add
3.0 mL
Concentration
~3,333 mcg/mL
Storage After Mixing
2–4 weeks at 2–8°C
Reconstitution Steps
  1. Wipe vial stopper with alcohol swab. Allow to dry.
  2. Draw 3.0 mL of bacteriostatic water.
  3. Add water slowly down the inside wall of the peptide vial. Do not aim at the powder.
  4. Roll or gently swirl the vial. Do not shake — shaking can damage the peptide.
  5. Solution should be clear. Discard if cloudy or discolored.
  6. Label with date and concentration. Refrigerate at 2–8°C.
Research Dosing Protocol
PhaseDoseVolume (3,333 mcg/mL)Frequency
Initiation100 mcg0.03 mL (3 units)Once daily
Titration150 mcg0.045 mL (~5 units)Once daily
Maintenance200 mcg0.06 mL (6 units)1–2× daily
Dose pre-bed or in fasted windows. Unlike CJC-1295 with DAC, this version has a short half-life (~30 min) mimicking natural pulsatile GH release.
🏋️

HGH — Human Growth Hormone

Recombinant HGH — anabolic signaling, lipolysis, IGF-1 · 15 IU Vial
Vial Size
15 IU
BAC Water to Add
1.5 mL
Concentration
10 IU/mL
Storage After Mixing
Up to 30 days at 2–8°C
Reconstitution Steps
  1. Bring the vial to room temperature (~10 min) before opening.
  2. Wipe stopper with an alcohol swab. Let dry.
  3. Draw 1.5 mL of bacteriostatic water into a sterile syringe.
  4. Insert needle and let water flow slowly down the side wall — never squirt directly onto the powder.
  5. Gently roll between palms until the lyophilized cake fully dissolves. Never shake or vortex.
  6. Solution should be clear and colorless. Discard if cloudy or white particles remain.
  7. Label with date and concentration (10 IU/mL). Refrigerate immediately at 2–8°C.
Research Dosing Protocol
Research RangeDoseVolume (10 IU/mL)Frequency
Low / introductory1–2 IU0.10–0.20 mL (10–20 units)Once daily
Moderate2–4 IU0.20–0.40 mL (20–40 units)Once daily or split AM/PM
Inject subcutaneously. Many protocols split the daily dose — half in the morning (fasted) and half post-workout. Rotate sites to avoid lipodystrophy.
Never freeze reconstituted HGH — this destroys the protein. Discard vials after 30 days regardless of remaining volume.
Metabolic & Mitochondrial
⚙️

MOTS-C

Mitochondria-derived peptide — AMPK activation, insulin sensitivity · 10mg & 40mg Vials
Vial Size
10 mg or 40 mg
BAC Water to Add
1.0 mL (10mg) / 3.0 mL (40mg)
Concentration
10 mg/mL (10mg vial) / 13.3 mg/mL (40mg vial)
Storage After Mixing
2–3 weeks at 2–8°C
Reconstitution Steps
  1. Inspect the vial for the peptide amount.
  2. Wipe both stoppers with alcohol swabs.
  3. Draw 1.0 mL BAC water for the 10mg vial, or 3.0 mL for the 40mg vial.
  4. Add water slowly by trickling it down the vial's inside wall. Avoid direct powder contact.
  5. Swirl gently in slow circles until dissolved. Do not shake.
  6. Check clarity — discard if cloudy or particulate. MOTS-C is more heat-sensitive than most peptides.
  7. Refrigerate at 36–46°F (2–8°C). Label with date.
Research Dosing Protocol
DoseVolume (10 mg/mL)FrequencyCycle
5 mg0.50 mL (50 units)2–3× per week4–8 weeks on, 4–8 weeks off
MOTS-C is considered more sensitive to heat and time than most peptides. Keep refrigerated at all times once reconstituted. Do not freeze reconstituted solution.
🔋

NAD+

Cellular energy coenzyme — DNA repair, sirtuin activation · 500mg & 1000mg Vials
Vial Sizes
500 mg / 1,000 mg
BAC Water to Add
5 mL (500mg) / 10 mL (1000mg)
Concentration
100 mg/mL
Storage After Mixing
14–30 days at 2–8°C
Reconstitution Steps
  1. Bring the lyophilized vial to room temperature (5–10 minutes).
  2. Sterilize both vial stoppers with separate alcohol swabs.
  3. Draw the planned BAC water volume into a sterile syringe (5 mL for 500mg, 10 mL for 1000mg).
  4. Inject water down the vial's interior wall to minimize foaming.
  5. Gently roll or swirl until the powder fully dissolves.
  6. Inspect for clarity — discard if discolored or cloudy.
  7. Label with concentration and date. Refrigerate at 2–8°C.
Research Dosing Protocol
RouteDoseVolume (100 mg/mL)Frequency
SubQ (starting)50 mg0.50 mL (50 units)2–3× per week
SubQ (maintenance)100 mg1.0 mL2–3× per week
Think of NAD+ like fuel for your cells — it helps them produce energy and repair themselves. Start at a lower dose and increase gradually as tolerated.
Neurological & Cognitive
🧠

PT-141 / Bremelanotide

Melanocortin MC3/MC4 receptor agonist — CNS neuro-hormonal pathways · 10mg Vial
Vial Size
10 mg
BAC Water to Add
2.0 mL
Concentration
5 mg/mL
Storage After Mixing
Refrigerate at 2–8°C
Reconstitution Steps
  1. Inspect the vial for integrity.
  2. Sterilize stoppers with alcohol swabs.
  3. Draw 2 mL of bacteriostatic water.
  4. Inject BAC water onto the inner wall of the vial — not directly onto the powder.
  5. Roll vial gently between palms until clear. Avoid shaking.
  6. Verify solution is clear and colorless. Refrigerate and label with date.
Research Dosing Protocol
DoseVolume (5 mg/mL)TimingFrequency
1.75 mg0.35 mL (35 units)45 min to 1 hr before activityNo more than 1× per 24 hrs / max 8× per month
Do not freeze reconstituted solution. Inject subcutaneously — abdomen or thigh. Nausea is common at higher doses; starting at lower amounts is advisable in research settings.
🧘

SELANK

Synthetic tuftsin analogue — anxiolytic, nootropic, immune modulation · 10mg Vial
Vial Size
10 mg
BAC Water to Add
2.0 mL
Concentration
5,000 mcg/mL
Storage After Mixing
3–4 weeks at 2–8°C
Reconstitution Steps
  1. Gather supplies: Selank vial, BAC water, U-100 insulin syringe, alcohol swabs.
  2. Clean both vial stoppers with separate alcohol swabs. Allow to air dry.
  3. Draw 2.0 mL of bacteriostatic water.
  4. Add water slowly to the Selank vial along the inside wall.
  5. Let sit 1–2 minutes, then gently roll between palms until clear. Shaking can damage the peptide.
  6. Label with date and concentration (5,000 mcg/mL). Refrigerate.
Research Dosing Protocol
PhaseDoseVolume (5,000 mcg/mL)Frequency
Weeks 1–2250–300 mcg0.05–0.06 mL (5–6 units)Once daily
Weeks 3–4400–500 mcg0.08–0.10 mL (8–10 units)Once daily
Weeks 5–8Off (rest period)——
Weeks 9–12300–500 mcg0.06–0.10 mL (6–10 units)Once daily
Also available as intranasal: 14–21 day course at 600–2,700 mcg daily split into 2–3 sprays. 1–3 week washout before repeating.
Do not freeze reconstituted solution. Avoid repeated freeze-thaw cycles.
💡

SEMAX

ACTH(4-7) analogue — neuroprotection, BDNF upregulation, cognition · 10mg Vial
Vial Size
10 mg
BAC Water to Add
2.0–3.0 mL
Concentration
3.3–5 mg/mL
Storage After Mixing
Several weeks at 2–8°C
Reconstitution Steps
  1. Inspect the vial for compound name and total mg.
  2. Wipe stoppers with separate alcohol swabs.
  3. For 5 mg/mL: draw 2.0 mL BAC water. For 3.3 mg/mL: draw 3.0 mL.
  4. Inject along the side wall at an angle — let water run down the vial interior rather than spraying directly onto powder.
  5. Swirl gently. Avoid vigorous shaking which can foam and stress the peptide.
  6. Inspect for clarity. Label and refrigerate with reconstitution date.
Research Dosing Protocol
RouteDoseVolume (3.3–5 mg/mL)Timing
Intranasal (primary)0.6–1.5 mg/day split in 20.06–0.15 mL per doseMorning + early afternoon
SubQ~0.5 mg per injection0.10 mL (10 units)Daily
Avoid dosing in the late afternoon/evening — Semax can be stimulating and may affect sleep.
Mitochondrial Support
🔬

SS-31 / Elamipretide

Cardiolipin-targeting tetrapeptide — mitochondrial membrane stabilization · 50mg Vial
Vial Size
50 mg
BAC Water to Add
2.5 mL
Concentration
20 mg/mL
Storage After Mixing
Up to 28 days at 2–8°C
Reconstitution Steps
  1. Inspect the vial for damage or contamination.
  2. Bring to room temperature (10–20 minutes) to prevent condensation.
  3. Wipe both stoppers with fresh alcohol swabs.
  4. Draw 2.5 mL of bacteriostatic water into a sterile syringe.
  5. Inject slowly down the vial wall to minimize foaming.
  6. Swirl gently (do not shake) until fully clear. Label with date and store refrigerated.
Research Dosing Protocol
DoseVolume (20 mg/mL)Frequency
40 mg2.0 mLOnce daily, same time each day
Do not freeze reconstituted solution. Protect from light. Discard after 28 days.
Sleep & Recovery
🌙

DSIP — Delta Sleep-Inducing Peptide

Neuropeptide — slow-wave sleep modulation & stress axis · 5mg Vial
Vial Size
5 mg
BAC Water to Add
1.0 mL
Concentration
5,000 mcg/mL
Storage After Mixing
Refrigerate at 2–8°C
Reconstitution Steps
  1. Wipe vial stopper with alcohol swab. Let dry.
  2. Draw 1.0 mL of bacteriostatic water.
  3. Inject BAC water slowly down the inner wall of the DSIP vial. Do not spray directly onto powder.
  4. Gently swirl until fully dissolved.
  5. Label with date and concentration. Refrigerate.
Research Dosing Protocol
Dose RangeVolume (5,000 mcg/mL)TimingCycle
100–200 mcg (lower)0.02–0.04 mL (2–4 units)1–3 hrs before sleep5–7 night cycles or 4-week cycle
200–300 mcg (higher)0.04–0.06 mL (4–6 units)1–3 hrs before sleep5–7 night cycles or 4-week cycle
Single evening subcutaneous injection 1–3 hours before intended sleep. Do not freeze reconstituted solution.
Supplies You'll Need
🧰

General Supplies Checklist

Everything needed for safe peptide reconstitution
Reconstitution Syringe
1 mL or 3 mL sterile syringe with 23-25g needle
Injection Syringe
U-100 insulin syringe (29-31g, ½ inch)
Bacteriostatic Water
Sterile BAC water for injection (30 mL vials)
Alcohol Swabs
70% isopropyl alcohol prep pads
Sharps Container
FDA-approved sharps disposal container
Storage
Refrigerator at 2–8°C (36–46°F)
General Rules: Always inject water down the side wall, never directly onto the powder. Always roll — never shake. Always inspect for clarity before use. When in doubt, discard. Keep all reconstituted peptides refrigerated and protected from light.